U.S. Federal Route 53 is a north-south highway in Northwestern La Crosse County. It runs from a junction with U.S. Rt. 14/61; Wisconsin State Route 16/33 in La Crosse to the La Crosse/Trempealeau County Line, near Galesville. U.S. Rt. 53 passes through the following towns in La Crosse County. La Crosse Onalaska