Purushottam Gawande, Ph. D.

Highlights of Qualification

Work Experience

Publications

Conference Poster Presentation

Research Experience

Education

Technical Skills

Teaching Experience

Research Interest

Awards

Computer Awareness

 

 

Home Address:

880 Bairdmore Blvd,

Winnipeg, MB, R3T 5G3

 

( (204) 255-7067

Email: gawandepv@yahoo.com

  

Highlights of Qualification

Ø Research Lab Experience in North America- 5 years

Ø      Biofilm, Food Microbiology, Molecular Biology and Native Enzymes

Ø      Conversant with conventional and automated microbiology techniques and strong computer skills

Ø      Exceptionally well organized, highly motivated

Ø      NSERC-IRF (2004) Canada, award for academic excellence and research ability

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WORK EXPERIENCE (INDUSTRIAL)

Kane Biotech Inc, Winnipeg, MB, Canada                                                April 2005

Position: Research Scientist

Responsibilities:

  • Develop biofilm, enzyme, enzyme kinetic, catheter adhesion assay

  • Design, write and review experimental protocols, SOPs, manuscripts for publications

WORK EXPERIENCE (ACADEMIC)

Canadian Research Institute for Food Safety (CRIFS),                                             University of Guelph, ON, Canada                                                                                     Positions: Special Graduate Faculty                          November 2004- March 2005         Post Doctorate                                                                July 2002 - March 2005                      Title of Project: Molecular-based models (MBM) for survival of Food Pathogens.

Responsibilities:  

  • Perform independent research on “Molecular-based models (MBM) for survival of Food Pathogens” and “Listeria and E. coli Biofilm”

  • Supervise M. Sc. Student research progress  

  • Writing reports, projects and manuscript for publications.

  • Responsible for teaching 

    • Rapid methods for separation and concentration of bacteria in foods  
    • Enumeration techniques current and future technology  

U. S. Department of Agriculture - Agriculture Research Services (USDA-ARS), Beltsville, MD, USA                                                                                                        Research Fellow                                                          January 2000- December 2001        Title of Project: To determine acid tolerance and growth of human pathogens on fresh-cut fruits and vegetables.

Responsibilities:

  • Perform independent research on ”To determine acid tolerance and growth of human pathogens on fresh-cut fruits and vegetables”

  • Supervise summer students

  • Writing reports and manuscripts for publications  

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PUBLICATIONS

BIOFILM

  1. Yakandawala, N., Gawande, P.V., LoVetri, K., & Madhyastha, S. (2006) Effect of ovotransferrin, protamine sulfate and EDTA combination on biofilm formation by catheter-associated bacteria. Submitted for Publication.

  2. Burton, E., Gawande, P.V., Yakandawala, N., LoVetri, K., Zhanel, G.G., Romeo, T., Friesen, A.D., & Madhyastha, S. (2006) Antibiofilm Activity of GlmU Enzyme Inhibitors Against Catheter-Associated Uropathogens. Antimicrobial Agent Chemotherapy In Press.

BACTERIAL PHYSIOLOGY/FOOD MICROBIOLOGY

  1. Gawande, P. V. & Griffiths, M. W. (2005) Growth History Influences Starvation-induced Expression of uspA, grpE and rpoS in Escherichia coli O157:H7 and their role in subsequent cryotolerance. Journal of Food Protection 68: 1154-1158.

  2. Gawande, P. V. & Griffiths, M. W. (2005) Effects of environmental stresses on the activities of the uspA, grpE and rpoS promoters of Escherichia coli O157:H7. International Journal of Food Microbiology 99: 91-98.

  3. Gawande, P. V. & Bhagwat, A. A. (2002) Protective effects of cold temperature and surface-contact on acid tolerance of Salmonella spp. Journal of Applied Microbiology 93: 689-696

  4. Gawande, P. V. & Bhagwat A. A. (2002) Inoculation onto certain solid surface protects Salmonella spp. during acid-challenge: A Model study using polyethersulphone membranes. Applied and Environmental Microbiology 68: 86-92

XYLANASES ENZYME/ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSE

  1. Gawande, P. V. & Kamat, M. Y.  (2000) Production of xylanases by immobilized Aspergillus sp. using lignocellulosic waste. World Journal of Microbiology and Biotechnology 16: 111-112

  2. Gawande, P. V. & Kamat, M. Y. (1999) Production of Aspergillus xylanases by lignocellulosic waste fermentation and its application. Journal of Applied Microbiology 87: 551-519.

  3. Gawande, P. V. & Kamat, M. Y. (1999) Purification of Aspergillus sp. xylanases by precipitation with an anionic polymer Eudragit S100. Process Biochemistry 34: 577-580.

  4. Gawande, P. V. & Kamat, M. Y. (1998) Preparation, characterization and application of Aspergillus sp. xylanase immobilized on Eudragit S100. Journal of Biotechnology 66: 165-175.

  5. Gawande, P. V. & Kamat, M. Y. (1998) Immobilization of Aspergillus sp. on nylon bolting cloth for production of xylanase. Journal of Fermentation and Bioengineering 86: 243-246.

PATENT

  1. Madhyastha, S. and Gawande, P.V. (2006) Dispersin B enzyme-based antimicrobial compositions for wound care devices. Provisional Patent Application Submitted.

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CONFERENCE POSTER PRESENTATION

  • Burton, E., Gawande P. V., Yakandawla, N., LoVetri, K., Zhanel, G.G., Romeo, T., Friesen, A.D., & Madhyastha, S. Antibiofilm Activity of GlmU Enzyme Inhibitors Against Catheter-Associated Uropathogens. International Center for Infectious Disease (ICID)-Research and Innovation Retreat, Winnipeg, MB, Canada, October 28-29, 2005.

  • Gawande, P. V. and Griffiths, M. W. Effect of growth media on starvation mediated expression of uspA::gfpuv, grpE::gfpuv and rpoS::gfpuv in Escherichia coli O157:H7 and their role in subsequent cryotolerance. Canadian Institute of Food Science and Technology & Agriculture and Agri-Food Canada joint conference (CIFST/AAFC 2004). University of Guelph, Guelph, ON, Canada. May 16-19, 2004.

    •

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RESEARCH EXPERIENCE

Project 1: Molecular-Based Models (MBM) for Survival of Food Pathogens               

  • Develop reports of stress responses by fusion of reporter genes, such as luciferase (lux) or green fluorescence protein (gfp) and promoter regions controlling particular stress responses.      

  • To study the physiological responses of foodborne microorganisms to various stress. [Details]

    •

Project 2: To determine acid tolerance and growth of human pathogens on fresh-cut fruits and vegetables.              

  • A model study on Salmonella acid tolerance in planktonic and polyethersulphone membrane attached cells was carried out.

  • Development of Salmonella gene array to study the Molecular and genetic analysis of attachment mediated acid shock [Details]

    •

Project 3: Studies In Microbial Xylanases             

  • Xylanases which has application in paper, pulp industry were immobilized and purified for first time using reversibly soluble-insoluble polymer Eudragit S 100..

  • Media development for xylanase production by solid-state cultivation of Aspergillus sp, immobilized on nylon bolting cloth for xylanases production. [Details]

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EDUCATION

 

Ph. D.  Microbiology,                                                                                          1999          University Institute of Chemical Technology (UICT)                                                              formerly University Department of Chemical Technology                                                                   Foods and Fermentation Technology                                                                               University of Mumbai                                                                                                            Thesis - Studies in Microbial Xylanases                                         

M. Sc. Microbiology                                                                                             1994     University of Poona

B . Sc. Microbiology                                                                                           1992    University of Poona

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TECHNICAL SKILLS

  • Expert in ll the routine and automated techniques of isolation, identification of microbial culture viable count, preservation of microbial pathogens using sterile technique, viable and dead cell count by differential staining.

  • Biofilm assay for aerobic and anaerobic cultures.

  • Proficient in techniques involved in isolation and purification of enzymes from microbial cultures using anionic polymer Eudragit S100 and characterization of proteins, 1D and 2D gel electrophoresis, Agarose gel electrophoresis, enzyme kinetic assay (estimation of Km and Vmax).

  • Isolation of genomic DNA, RNA, PCR, RT-PCR, transformation.

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TEACHING EXPERIENCE

 

       Responsible for teaching at the University of Guelph

  • Rapid method for separation and concentration of bacteria in foods

  • Enumeration techniques current and future technology

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RESEARCH INTEREST

  • Bacterial physiology

  • Microbial quality of food

  • Rapid methods of pathogen identification

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AWARDS

 

  • Natural Science and Engineering Research Council of Canada - Industrial Research Fellowship (NSERC-IRF), 2004- This award recognizes academic excellence, research ability and potential, and leadership and communication skills.

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COMPUTER AWARENESS

 

      Text editor, spreadsheet applications and presentation software. PC in DOS/WINDOWS  environment, sigma plot, Sigma Stats, Pubmed, Chemdraw.

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