The majority of name brand pregnancy tests require at least 50-100 miu of HCG to produce a positive result. Therefore, it is wise to wait until you have missed your period before testing. If you test before, you could get a false-negative when in fact you are pregnant, you just haven't produce enough hormone. A blood test (or serum test) acts in the same way as a urine test, however, pregnancy can be determined much earlier. The pregnancy hormone, HCG, can be detected as early as 7-10 days after conception using a blood test.
If you have all or some of the symptoms of pregnancy and still have a negative result, please see a doctor as soon as possible. Your baby is depending on you.